Identification of Habrobracon hebetor populations with RAPD markers

It was set that food preferences and terms of breeding of Habrobracon hebetor laboratory populations vary considerably. In this connection the necessity of intraspecies differentiations of populations gets up. Conducting identification of populations is today possible with DNA markers that are only effective and reliable means for the estimation of genetic distinctions between the populations of this insect species. RAPD analysis of different geographic populations of H.hebetor (Krasnodar, Russia and Chimkent, Kazakhstan) was conducted. 21 RAPD primers were tested on specificity to H.hebetor DNA. There were reviled five RAPD primers (ОРА05, ОРА10, ОРВ01, ОРВ04, UBC519), with high specificity and ability to different H.hebetor populations. DNA markers specific to Krasnodar and Chimkent populations were conducted. They use clearly identify these H.hebetor populations. For the Krasnodar population there were RAPD- markers: 550 b.p. (UBC519), 500 and 700 b.p. (ОРА05); 1100, 1200 and 1300 b.p. (ОРА10); 220 and 800 b.p. (ОРВ04); 880 b.p. (ОРВ01); for the Chimkent population: 400, 600 and 1200 b.p. (UBC519); 600 and 950 b.p. (ОРА10); 800 b.p. (ОРВ01). Fully probably, that these RAPD primers can be used for identification and differentiation of other populations of H.hebetor.

Keywords

PCR, RAPD analysis, primer, insects, Habrobracon hebetor, population, DNA polymorphism, genetic diversity

Received

17.08.2018

Publication date

30.09.2018

Number of characters

1282

Cite Download pdf To download PDF you should sign in

GOST	Kil V., Agasieva I., Besedina E., Balaban A., Ismailov V. Identification of Habrobracon hebetor populations with RAPD markers // Rossiiskaia selskokhoziaistvennaia nauka – 2018. – Number 4 C. 36-39 [Electronic resource]. URL: http://ras.jes.su/rshn/s207987840000481-8-1-en (circulation date: 25.01.2025). DOI: 10.31857/S250026270000546-5
MLA	Kil, V., Agasieva, I., Besedina, E., Balaban, A., Ismailov, V. "Identification of Habrobracon hebetor populations with RAPD markers." Rossiiskaia selskokhoziaistvennaia nauka 4 (2018):36-39. DOI: 10.31857/S250026270000546-5
APA	Kil V., Agasieva I., Besedina E., Balaban A., Ismailov V. (2018). Identification of Habrobracon hebetor populations with RAPD markers. Rossiiskaia selskokhoziaistvennaia nauka (4), pp.36-39 DOI: 10.31857/S250026270000546-5

Previous versions

S250026270000546-5-1 Дата внесения правок в статью - 30.09.2018

Размещенный ниже текст является ознакомительной версией и может не соответствовать печатной

Readers community rating: votes 0

1. Ismailov V.Ya., Agas'eva I.S., Kil' V. I., Fedorenko E. V., Besedina E. N., Nefedova M. V. Izuchenie biologicheskikh osobennostej ehktoparazita HabrobraconhebetorSay v tselyakh optimizatsii biotsenoticheskoj regulyatsii chislennosti vrednykh cheshuekrylykh // Nauka Kubani. – 2017. – № 4. – S. 26-33.

2. Agas'eva I.S., Ismailov V.Ya., Kil' V.I., Fedorenko E.V., Besedina E.N., Kuvika T.O., Nefedova M.V. Izuchenie biologicheskikh osobennostej populyatsij ehktoparazita Habrobraconhebetor SAY (Hymenoptera, Braconidae) dlya biologicheskoj zaschity rastenij // Materialy Mezhd. nauchno-prakticheskoj konf. «Biologicheskaya zaschita rastenij - osnova stabilizatsii agroehkosistem» s molodezhnoj strategicheskoj sessiej «Kadry, resursy, vozmozhnosti, innovatsii». Vypusk 9, 20-22 sentyabrya 2016 g., Krasnodar, S.106-111.

3. Williams J.G.K., Kubelik A.R., Livak K.J. et al. DNA polymorphism’s amplified by arbitrary primers are useful as genetic markers // Nucl. AcidsRes. 1990. – V. 18. – P. 6531-6535.

4. Kil' V.I. Ispol'zovanie vysokospetsificheskikh RAPD-prajmerov dlya PTsR-analiza populyatsij vrednykh i poleznykh nasekomykh // Doklady Rossel'khozakademii. – 2014. – №6. – S.21-25.

5. Antolin M. F., Bosio C. F., Cotton J., Sweeney W., Strand M. R., Black W.C. Intensive Linkage Mapping in a Wasp (Bracon hebetor) and a Mosquito(Aedes aegypti) With Single-Strand Conformation PolymorphismAnalysis of Random Amplified Polymorphic DNA Markers // Genetics 145. (August, 1996). – P. 1727-1738.

6. Holloway A.K., Heimpel G. E., Strand M.R.,Antolin M.F. Survival of Diploid Males in Bracon sp. near hebetor(Hymenoptera: Braconidae) // Ann. Entomol. Soc. Am. 92(1). – 1999. – P.110-116.

7. Holloway A.K., Strand M.R., Black W.C. IV, Antolin M.F. Linkage Analysis of Sex Determination in Bracon sp. Near hebetor (Hymenoptera: Braconidae) // Genetics 154. (January 2000). – P.205–212.

8. Antolin MF, Ode PJ, Heimpel GE, O’Hara RB, Strand MR. Population structure, mating system, and sexdeterminingallele diversity of the parasitoid wasp Habrobracon hebetor // Heredity.– 2003. – 91. – R.373–381.

9. Kil' V.I., Besedina E.N., Agas'eva I.S., Ismailov V.Ya. DNK polimorfizm i geneticheskoe raznoobrazie krasnodarskoj populyatsii Habrobracon hebetor // Wschodnioeuropejskie Czasopismo Naukowe (East European Scientific Journal). – 2016. – N 12. – P.49-51.

10. Kil' V.I., Besedina E.N., Agas'eva I.S., Ismailov V.Ya. RAPD-analiz laboratornoj i prirodnoj populyatsij Habrobracon hebetor // Scientific symposium (IV-th edition) «Advanced Biotechnologies - Achievements and Prospects, in October 3-4, 2016 in Chisinau, Republic of Moldova, P.11.

11. Kil' V.I., Balaban A.T. PTsR-analiz ehktoparazita Habrobraconhebetor po RAPD- i ISSR-markeram // V sbornike: Sovremennye problemy nauki, tekhnologij, innovatsionnoj deyatel'nosti. Sbornik nauchnykh trudov po materialam Mezhd. nauchno-prakticheskoj konf. V 4-kh chastyakh. / Pod obschej redaktsiej E.P. Tkachevoj. – Belgorod, 2017. – S. 18-20.K

12. il' V.I. Metodika otsenki DNK polimorfizma populyatsij nasekomykh s pomosch'yu PTsR (RAPD- i ISSR-PCR) // Metodicheskie rekomendatsii. – Krasnodar: OOO «Prosveschenie-Yug», 2009. – 16 s.

13. Van de Peer, Y., De Wachter, R. Construction of evolutionary distance trees with TREECON for Windows: accounting in nucleotide substitution rate among sites. // Comput. Applic. Biosci. – 1997. – 13. – P.227-230.

14. Yeh F.C. Yang R.C., Boyle T.B.J., Ye Z.H., Mao J.X. POPGENE, the user-friendly shareware for population genetic analysis. Computer program and documentation // Molecular Biology and Biotechnology Centre. University of Alberta, Edmonton, 1997.