Influence of time of cultivation of Staphylococcus aureus on the production of cytokine-like substances that detected by ELISA

 
PIIS102872210002427-4-1
DOI10.31857/S102872210002427-4
Publication type Article
Status Published
Authors
Affiliation: Institute of Immunology and Physiology UrB RAS
Affiliation:
Institute of Immunology and Physiology UrB RAS
South-Ural State University (National Research University)
Affiliation: State research Institute of high-purity biological products
Affiliation: Institute of Cellular and Intracellular Symbiosis UrB RAS
Journal nameRossiiskii immunologicheskii zhurnal
EditionVolume 12 Issue 3
Pages454-459
Abstract

The paper presents the results of the study of supernatants 24 and 48 hours cultures of various strains of Staphylococcus aureus by the method of enzyme immunoassay (ELISA) by means of test-systems for determining the levels of human cytokines INF-α, INF-γ, IL-1Ra, IL-1β, IL-4, IL-6, IL-8, IL-10, IL-17, TNF-α (“Cytokine”, St. Petersburg). It has been established that a number of strains of staphylococci are able to secrete various cytokine-like substances (CLSs), determined by test systems for ELISA, in the incubation medium. The highest rates were established for CLSs, “identical” to human cytokines INF-γ, INF-α, IL-4 and IL-1Ra. The remaining CLSs were determined in minimum concentrations. At the same time, the level of bacterial CLSs that was detected in high concentrations signifi cantly increased signifi - cantly with increasing incubation time of S. aureus from 24 to 48 hours.

Keywordscytokines, S. aureus, cytokine-like substances
AcknowledgmentThe work was done on the topic from the Plan of Research and Development IIF UB RAS, № state. the registration of AAAA-A18–118020690020–1, and the topic from the Plan of R & D of ICWS, UB RAS, No. of State. registration number 116021510075.
Received12.01.2019
Publication date12.01.2019
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