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Dependence of neutrophiles respiratory burst from the state of their metabolic processes in patients with chronic endometritis and adnexitis
 
PIIS102872210002420-7-1
DOI10.31857/S102872210002420-7
Publication type Article
Status Published
Authors
A. Savchenko 
Affiliation: Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences
E. Kasparov
Affiliation: Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences
S. Arutjunjan
Affiliation: Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences
A. Borisov
Affiliation: Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences
I. Gvozdev
Affiliation: Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences
Journal nameRossiiskii immunologicheskii zhurnal
EditionVolume 12 Issue 3
Pages412-422
Abstract

The purpose of this work was to study the dependence of neutrophils respiratory burst from the activity of NAD(P)-dependent dehydrogenases in patients with chronic endometritis and adnexitis. The study involved 89 women of reproductive age with chronic endometritis and adnexitis. As a control were examined 98 healthy women of a similar age range. The respiratory burst condition of blood neutrophils was examined using a chemiluminescent assay. Investigation of the NAD(P)-dependent dehydrogenases activity in neutrophils was carried out using the bioluminescent method. A high intensity of the respiratory explosion of neutrophils was detected in chronic endometritis that was determined by an increase in the synthesis of primary and secondary reactive oxygen species (ROS). In patients with chronic adnexitis a respiratory burst of neutrophils occurs in the imbalance of synthesis of primary and secondary ROS: with a reduced activity of NADPH-oxidase there was a high level of secondary ROS synthesis. Metabolism of neutrophils in women with infectious and infl ammatory diseases of the pelvic organs is characterized by a high level of lipid metabolism and NAD-dependent substrate fl ow between the reactions of the tricarboxylic acid cycle and the amino acid exchange. A feature of neutrophil metabolism in chronic endometritis is the high activity of malate dehydrogenase. Characteristic features of neutrophil granulocyte metabolism in patients with chronic adnexitis are a decrease in the intensity of anaerobic glycolysis and NADP-dependent substrate fl ow between the reactions of the tricarboxylic acid cycle and the amino acid exchange. The relationship between respiratory explosion rates and neutrophil metabolism in chronic endometritis refl ects the inhibitory eff ect of the anaerobic reaction of lactate dehydrogenase on the induced synthesis of primary ROS. The respiratory explosion of neutrophils in women with chronic adnexitis depends on the activity of NADP-dependent dehydrogenases, but with the pronounced eff ect of enzymes in the energy profi le. A characteristic feature of chronic adnexitis also is a stimulating eff ect on the synthesis of primary ROS activity of glutathione reductase. Determination of the metabolic regulation mechanisms of respiratory burst in neutrophils allows us to characterize the regulatory aspects of immunopathogenesis of infectious and infl ammatory diseases and can be the basis for the development of new methods of immunometabolic therapy.

Keywordschronic endometritis, chronic adnexitis, neutrophils, respiratory burst, metabolism, dehydrogenase activity
Received11.01.2019
Publication date12.01.2019
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1. Borisov A. G., Savchenko A. A., Sokolovskaya V. K. Zabolevaemost', svyazannaya s narusheniyami funktsii immunnoj sistemy (na primere Krasnoyarskogo kraya). Zdravookhranenie Rossijskoj Federatsii 2014, 58(6), 38–41. [Borisov A. G., Savchenko A. A., Sokolovskaya V. K. The morbidity related to disorders of immune system function as exemplifi ed by the Krasnoyarsk region. Healthcare of the Russian Federation 2014, 58(6), 38–41. Russian].
2. Wadhwa N., Raoot A. An unusual case of adult fi larial oophoritis. International Society of Gynecological Pathologists 2011, 30(6), 549–552.
3. Savchenko A. A., Kasparov Eh. V., Arutyunyan S. S., Borisov A. G., Kudryavtsev I. V., Moshev A. V. Fenotipicheskij sostav V-limfotsitov u zhenschin s khronicheskim ehndometritom i adneksitom. Meditsinskaya immunologiya 2016, 18(4), 379–384.[Savchenko A. A., Kasparov E. V., Arutyunyan S. S., Borisov A. G., Kudryavtsev I. V., Moshev A. V. Phenotypic profi le of B-lymphocytes in women with chronic endometritis and adnexitis. Medical Immunology (Russia) 2016, 18(4), 379–384. Russian].
4. Yasui T., McCann K., Gilbert R. O., Nydam D. V., Overton T. R. Associations of cytological endometritis with energy metabolism and infl ammation during the periparturient period and early lactation in dairy cows. American Dairy Science Association 2014, 97(5), 2763–2770.
5. Savchenko A. A., Zdzitovetskij D. Eh., Borisov A. G. Immunometabolicheskie narusheniya pri rasprostranennom gnojnom peritonite. Novosibirsk: Nauka, 2013, 142 s. [Savchenko A. A., Zdzitoveck? D. Je., Borisov A. G. The immune and metabolic disorders by the widespread purulent peritonitis. Novosibirsk: Nauka, 2013, 142 p. Russian].
6. Lavoie-Lamoureux A., Moran K., Beauchamp G., Mauel S., Steinbach F., Lefebvre-Lavoie J., Martin J. G., Lavoie J. P. IL-4 activates equine neutrophils and induces a mixed infl ammatory cytokine expression profi le with enhanced neutrophil chemotactic mediator release ex vivo. American journal of physiology. Lung cellular and molecular physiology 2010, 299(4), L472–L482.
7. Pham Van L., Germaud N., Ramadan A., Thieblemont N. MyD88 modulates eosinophil and neutrophil recruitment as well as IL-17A production during allergic infl ammation. Cellular immunology 2016, 310, 116–122.
8. Tan S. Y., Weninger W. Neutrophil migration in infl ammation: intercellular signal relay and crosstalk. Current opinion in immunology 2016, 44, 34–42.
9. Savchenko A. A., Zdzitovetskij D. Eh., Borisov A. G., Luzan N. A. Khemilyuminestsentnaya aktivnost' nejtrofil'nykh granulotsitov i urovni kontsentratsii tsitokinov u bol'nykh rasprostranennym gnojnym peritonitom. Tsitokiny i vospalenie 2013, 12(1– 2), 115–119. [Savchenko A. A., Zdzitovec k? D. Je., Borisov A. G., Luzan N. A. Chemiluminescent activity of neutrophilic granulocytes and cytokine concentrations in patients with advanced purulent peritonitis. Cytokines and infl ammation 2013, 12(1–2), 115–119. Russian].
10. Shkapova E. A., Kurtasova L. M., Savchenko A. A. Poka- zateli lyutsigenin- i lyuminol-zavisimoj khemilyu-minestsentsii nejtrofilov krovi u bol'nykh rakom pochki. Byulleten' ehksperimental'noj biologii i meditsiny 2010, 149(2), 201–203. [Shkapova E. A., Kurtasova L. M., Savchenko A. A. The indicatours of the lucigenin- and luminol-dependent chemiluminescence of blood neutrophils by the patients with kidney cancer. Bulletin of Experimental Biology and Medicine 2010, 149(2), 201–203. Russian].
11. Li H., Zhao Y., Li W., Yang J., Wu H. Critical role of neutrophil alkaline phosphatase in the antimicrobial function of neutrophils. Life sciences 2016, 157, 152– 157.
12. McGovern N.N., Cowburn A. S., Porter L., Walmsley S. R., Summers C., Thompson A. A., Anwar S., Willcocks L. C., Whyte M. K., Condliff e A. M., Chilvers E. R. Hypoxia selectively inhibits respiratory burst activity and killing of Staphylococcus aureus in human neutrophils. The Journal of immunology 2011, 186(1): 453– 463.
13. Sun D., Crowell S. A., Harding C. M., De Silva P. M., Harrison A., Fernando D. M., Mason K. M., Santana E., Loewen P. C., Kumar A., Liu Y. KatG and KatE confer Acinetobacter resistance to hydrogen peroxide but sensitize bacteria to killing by phagocytic respiratory burst. Life sciences 2016, 148, 31–40.
14. Savchenko A. A. Opredelenie aktivnosti NAD(P)-zavisimykh degidrogenaz v nejtrofil'nykh granulotsitakh biolyuminestsentnym metodom. Byulleten' ehksperimental'noj biologii i meditsiny 2015, 159(5), 656–660. [Savchenko A. A. Evaluation of NAD(P)-Dependent Dehydrogenase Activities in Neutro-philic Granulocytes by the Bioluminescent Method. Bulletin of Experimental Biology and Medicine 2015, 159(5), 692–695. Russian].
15. Azevedo E. P., Rochael N. C., Guimaraes-Costa A.B., de Souza-Vieira T.S., Ganilho J., Saraiva E. M., Palhano F. L., Foguel D. A Metabolic Shift toward Pentose Phosphate Pathway Is Necessary for Amyloid Fibril- and Phorbol 12-Myristate 13-Acetate-induced Neutrophil Extracellular Trap (NET) Formation. The Journal of biological chemistry 2015, 290(36), 22174–22183.
16. Kazumura K., Sato Y., Satozono H., Koike T., Tsuchiya H., Hiramatsu M., Katsumata M., Okazaki S. Simultaneous monitoring of superoxides and intracellular calcium ions in neutrophils by chemiluminescence and fl uorescence: evaluation of action mechanisms of bioactive compounds in foods. Journal of pharmaceutical and biomedical analysis 2013, 84, 90–96.
17. Rezende F., Prior K. K., Lowe O., Wittig I., Strecker V., Moll F., Helfi nger V., Schnutgen F., Kurrle N., Wempe F., Walter M., Zukunft S., Luck B., Fleming I., Weissmann N., Brandes R. P., Schroder K. Cytochrome P450 enzymes but not NADPH oxidases are the source of the NADPH-dependent lucigenin chemiluminescence in membrane assays. Free radical biology and medicine 2017, 102, 57–66.
18. Douillard S., Rozec B., Bigot E., Aillet L., Patrice T. Secondary reactive oxygen species production after PDT during pulmonary tumor growth in sera of nude mice. Photodiagnosis and photodynamic therapy 2013, 10(1), 62–71.
19. Trujillo M. Analysis of the lmmunity-Related Oxidative Bursts by a Luminol-Based Assay. Methods in molecular biology 2016, 1398, 323–329.
20. Balteau M., Tajeddine N., de Meester C., Ginion A., Des Rosiers C., Brady N. R., Sommereyns C., Horman S., Vanoverschelde J. L., Gailly P., Hue L., Bertrand L., Beauloye C. NADPH oxidase activation by hyperglycaemia in cardiomyocytes is independent of glucose metabolism but requires SGLT1. Cardiovascular research 2011, 92(2), 237–246.
21. Cai T., Kuang Y., Zhang C., Zhang Z., Chen L., Li B., Li Y., Wang Y., Yang H., Han Q., Zhu Y. Glucose-6- phosphate dehydrogenase and NADPH oxidase 4 control STAT3 activity in melanoma cells through a pathway involving reactive oxygen species, c-SRC and SHP2. American journal of cancer research 2015, 5(5), 1610–1620.
22. Sato T., Morita A., Mori N., Miura S. The role of glycerol- 3-phosphate dehydrogenase 1 in the progression of fatty liver after acute ethanol administration in mice. Biochemical and biophysical research communications 2014, 444(4), 525–530.
23. Liu L., Shah S., Fan J., Park J. O., Wellen K. E., Rabinowitz J. D. Malic enzyme tracers reveal hypoxia-induced switch in adipocyte NADPH pathway usage. Nature chemical biology 2016, 12(5), 345–352.
24. Bao Y., Ledderose C., Seier T., Graf A. F., Brix B., Chong E., Junger W. G. Mitochondria regulate neutrophil activation by generating ATP for autocrine purinergic signaling. The Journal of biological chemistry 2014, 289(39), 26794–26803.
25. Plaitakis A., Kalef-Ezra E., Kotzamani D., Zaganas I., Spanaki C. The Glutamate Dehydrogenase Pathway and Its Roles in Cell and Tissue Biology in Health and Disease. Biology (Basel) 2017, 6(1), E11.
26. Zhu M., Fang J., Zhang J., Zhang Z., Xie J., Yu Y., Ruan J. J., Chen Z., Hou W., Yang G., Su W., Ruan B. H. Biomolecular Interaction Assays Identifi ed Dual Inhibitors of Glutaminase and Glutamate Dehydrogenase That Disrupt Mitochondrial Function and Prevent Growth of Cancer Cells. Analytical chemistry 2017, 89(3), 1689–1696.

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