Identification and analysis of VTC2 homologs encoding key enzyme of L-ascorbic acid biosynthesis in tomato species (Solanum section of Lycopersicon)

 
PIIS086956520003457-5-1
DOI10.31857/S086956520003457-5
Publication type Article
Status Published
Authors
Affiliation: Institute of Biochemistry, RAS
Address: Russian Federation
Affiliation:
Institute of Biochemistry, RAS
Lomonosov Moscow State University
Address: Russian Federation
Affiliation:
Institute of Biochemistry, RAS
Lomonosov Moscow State University
Address: Russian Federation
Journal nameDoklady Akademii nauk
EditionVolume 483 Issue 6
Pages682-686
Abstract

The complete nucleotide sequences of 11 wild and cultivated tomato GDP-L-galactose-phosphorylase-1 homologs (VTC2) were identified and characterized. The overall level of nucleotide variability was 9.19%. Totally 25 out of 54 cDNA SNPs, were non-synonymous and resulted in amino acid substitutions. In the group of green-fruited tomato species, more SNPs were detected than in the group of red-fruited species, but the percentage of non-synonymous substitutions in red-fruited tomatoes was significantly higher (66.7% vs. 37.5%). In the translated amino acid sequences the GDF-L-galactose-hexose-1-phosphate guanyltransferase domain and highly conserved HIT-motif were identified and a conservative motif specific for VTC2 of Solanoideae species was detected.

Keywords
Received26.12.2018
Publication date26.12.2018
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