Use of biotechnological techniques in black currant reproduction

Currently, methods for the culture of isolated tissues and organs have been widely used to solve theoretical and applied problems of biotechnology. The article discusses the theoretical aspects of the use of biotechnological techniques in the reproduction of black currants (Ribes nigrum L.). The need for the use of biotechnological methods in the rapid reproduction of various forms and varieties of black currants due to the difficulty of reproduction of a number of genotypes by traditional methods, especially forms obtained using interspecific hybridization. Among the emerging issues are the following: find donors of valuable traits with the aim of increasing ecological plasticity of cultivated plants; a study of the influence of qualitative and quantitative composition of nutrient media for the cultivated plants, optimization of the timing of the introduction of plants and selection of optimal modifications of culture media with the varietal characteristics at different stages of cultivation; the study of the influence of hormonal and other biologically active substances on development of plants in conditions of culture in vitro. In the article literary sources, which describe those additional issues relating to process optimization clonal micropropagation of black currant. All stages of cultivation of the given culture in conditions in vitro from initiation to adaptation in non-sterile conditions are considered. The factors that determine the success of reproduction are described: terms of introduction into the culture and types of explants, selection of sterilizing agent, optimization of the nutrient medium depending on the passage, the influence of different components of the medium on regenerative plants. The methods of microclonal reproduction, developed by scientists of leading research organizations, data on ways to combat phenolic oxidation of the nutrient medium.

Keywords

black currant, clonal micropropagation, explants, morphogenesis, autoclaves, nutrient solution, regeneration

Received

06.12.2018

Publication date

06.12.2018

Number of characters

1781

Cite Download pdf To download PDF you should sign in

GOST	Khromova T., Tashmatova L., Matsneva O., Shakhov V. Use of biotechnological techniques in black currant reproduction // Vestnik of The Russian agricultural science – 2018. – №6-2018 C. 69-73 [Electronic resource]. URL: http://ras.jes.su/vrsn/s250020820002932-5-1-en (circulation date: 20.04.2024). DOI: 10.30850/vrsn/2018/6/69-73
MLA	Khromova, T., Tashmatova, L., Matsneva, O., Shakhov, V. "Use of biotechnological techniques in black currant reproduction." Vestnik of The Russian agricultural science 6 (2018):69-73. DOI: 10.30850/vrsn/2018/6/69-73
APA	Khromova T., Tashmatova L., Matsneva O., Shakhov V. (2018). Use of biotechnological techniques in black currant reproduction. Vestnik of The Russian agricultural science (6), pp.69-73 DOI: 10.30850/vrsn/2018/6/69-73

Размещенный ниже текст является ознакомительной версией и может не соответствовать печатной

Readers community rating: votes 0

1. Knyazeva, I.V. Osobennosti razvitiya ehksplantov yagodnykh rastenij na ehtape vvedeniya v kul'turu in vitro / I.V Knyazeva // Nauchnyj al'manakh – Tambov, 2015. – №10-3 (12) – S. 400-403.

2. Matushkin, S.A. Vliyanie askorbinovoj kisloty na regeneratsionnuyu sposobnost' izolirovannykh tkanej smorodiny chernoj i maliny / S.A. Matushkin, L.V. Yarmolenko // Plodovodstvo i yagodovodstvo Rossii: sb. nauch. rabot / M: VSTISP.– 2016.- T. KhKhKhKhVI. – S. 223 – 227.

3. Matushkin, S.A. Vliyanie mineral'nogo sostava sredy na regeneratsiyu izolirovannykh ehksplantov smorodiny chernoj i kryzhovnika in vitro / S.A. Matushkin // Plodovodstvo i yagodovodstvo Rossii: sb. nauch. rabot / M: VSTISP.– 2016. – T. KhKhKhKhIIII. – S. 192-197.

4. Metodicheskie rekomendatsii po ispol'zovaniyu biotekhnologicheskikh metodov v rabote s plodovymi, yagodnymi i dekorativnymi kul'turami. – Orel: VNIISPK. – 2005 – 51 s.

5. Razmnozhenie plodovykh rastenij v kul'ture in vitro / N.V. Kukharchik [i dr.]; pod obsch. red. N.V. Kukharchik. – Minsk: Belaruskaya navuka. – 2016. – 208 s.

6. Rajkov, I.A. Sovershenstvovanie klonal'nogo mikrorazmnozheniya mezhvidovykh form smorodiny chernoj i maliny remontantnogo tipa: avtoref. dis…. kand. s.-kh. n. /I.A. Rajkov – Bryansk. – 2012. – 19 s.

7. Rajkov, I.A Optimizatsiya razmnozheniya smorodiny chernoj v usloviyakh in vitro / I.A. Rajkov, D.N. Skovorodnikov, F.F. Sazonov // Biologizatsiya zemledeliya v Nechernozemnoj zone Rossii: Sbornik nauchnykh trudov Mezhd. nauchn.-prakt. konf., posvyaschennoj 30-letiyu Bryanskoj GSKhA i 70-letiyu so dnya rozhdeniya Zasluzhennogo deyatelya nauki RF, doktora s.- kh.n., professora V.F. Mal'tseva. – 2010. – S. 314-319.

8. Skovorodnikov, D.N. Nekotorye aspekty ispol'zovaniya tsitokininov razlichnoj prirody na ehtape vvedeniya v kul'turu in vitro ehksplantov chernoj smorodiny. / D.N. Skovorodnikov, I.A. Rajkov // Plodovodstvo i yagodovodstvo Rossii. – 2009. – T. XXII. – № 2. – S. 292-296.

9. Skovorodnikov, D.N. Osobennosti klonal'nogo mikrorazmnozheniya smorodiny chernoj. / D.N. Skovorodnikov, F.F. Sazonov // Plodovodstvo i yagodovodstvo Rossii: Sb. nauchnykh rabot. GNU VSTISP Rosselkhoz akademii. – 2011. – T. XXVI. – S. 395-400.

10. Skovorodnikov, D.N. Klonal'noe mikrorazmnozhenie v uskorenii selektsionnogo protsessa smorodiny chernoj / D.N. Skovorodnikov // Nauchnye vedomosti Belgorodskogo gosudarstvennogo universiteta. Seriya: Estestvennye nauki. – 2012. – T. 21. – №21-1 (140). – S. 58- 61.

11. Shakhov, V.V. Sravnitel'naya kharakteristika srokov vvedeniya ehksplantov chernoj smorodiny (Ribes nigrum L.) v kul'turu in vitro. / V.V. Shakhov, L.V. Tashmatova, O.V. Matsneva // Sovremennoe sadovodstvo – Contemporary horticulture – 2017, №4 (URL: http://journal.vniispk.ru/pdf/2017/4/39.pdf, data obrascheniya: 26.03.2018 g.).