The article presents and analyzes data of competitive varietal testing of promising varieties and lines of spring barley for yield and brewing qualities. Field studies were conducted in 2014–2017 on dark gray forest heavy loam soil. Agrochemical parameters are total nitrogen – 0.24%, humus content in a layer of 0-40 cm (according to Tyurin) – 5.19%, hydrolysis nitrogen – 123.5 mg / kg, salt extract pH – 4.92 mg-eq / 100g; labile phosphorus - 34.6 mg / 100g, labile potassium – 20.0 mg / 100g. The forerunner is winter wheat. Meteorological conditions in the years of research differed from each other and from the average long-term value. Barley samples were assessed by the protein content in the grain (GOST 10846-91), extract content (GOST 12130-77), weight 1000 grains (GOST 10842- 89). Ecological plasticity was determined by the method proposed by E.D. Nettevich, A.I. Morgunov and M.I. Maksimenko, stability index (Ľ) by A. A. Gryaznov, indicator of stability level (Puss) by E. D. Nettevich and A. I. Morgunov. The main measure for assessing quality indicators is protein content. Many other biochemical and technological features of grain depend on its level. The experimental data convincingly testify to the significant influence of the soil and climatic conditions on the yield and, especially, on the brewing qualities of barley in the conditions of the Central Region of the Nonchernozem Zone. According to the studied traits, new valuable varieties Nadezhny, Sir, Noble and selection lines 141 / 1-09 h 746, 23 / 1-10 h 784, distinguished by high adaptability and resistance to adverse environmental factors, have been identified.