Multipurpose utilization of the aspergillus oryzae fungus - the producer of the hydrolase complex

 
PIIS250020820001981-9-1
DOI
Publication type Article
Status Published
Authors
Occupation: Academician of RAS
Affiliation: All-Russian Research Institute of Food Biotechnology is a Branch of Federal Research Center of Food, Biotechnology and Food Safety
Address: Russian Federation
Occupation: Grand PhD in Biological sciences, professor
Affiliation: All-Russian Research Institute of Food Biotechnology is a Branch of Federal Research Center of Food, Biotechnology and Food Safety
Address: Russian Federation
Occupation: Overchenko, PhD in Technical sciences
Affiliation: All-Russian Research Institute of Food Biotechnology is a Branch of Federal Research Center of Food, Biotechnology and Food Safety
Address: Russian Federation
Occupation: Grand PhD in Technical sciences, professor
Affiliation: All-Russian Research Institute of Food Biotechnology is a Branch of Federal Research Center of Food, Biotechnology and Food Safety
Address: Russian Federation
Occupation: PhD in Technical sciences
Affiliation: All-Russian Research Institute of Food Biotechnology is a Branch of Federal Research Center of Food, Biotechnology and Food Safety
Address: Russian Federation
Occupation: PhD in Biological sciences
Affiliation: All-Russian Research Institute of Food Biotechnology is a Branch of Federal Research Center of Food, Biotechnology and Food Safety
Address: Russian Federation
Occupation: PhD in Biological sciences
Affiliation: All-Russian Research Institute of Food Biotechnology is a Branch of Federal Research Center of Food, Biotechnology and Food Safety
Address: Russian Federation
Journal nameVestnik of The Russian agricultural science
Edition№5-2018
Pages29-33
Abstract

The possibilities of multi-purpose use of metabolites Aspergillus oryzae 01134 under deep cultivation and residual biomass of micromycetes were investigated. By 36 h of growth of the fungus, the level of proteolytic activity (PS) in the culture fluid (CS) was 87%, and β-glucanase (GCS) – 97% of the maximum value achieved at 48 hours of growth. The results of studies of the dynamics of changes in the content of proteins and polysaccharides in the biomass of the fungus during its growth showed a decrease in their level to 48 h of growth, by 15.0; 5.0%, respectively, due to the increased activity of intracellular hydrolytic enzymes and the beginning of autolytic processes. Culture liquid was applied for filtration for biomass separation, ultrafiltration and drying to obtain a concentrated enzyme preparation (CFP). The enzyme preparation CFP with proteolytic activity of 680 u. PS/g, β-glucanase activity of -143 HLA/g, amylase activity of 764 AC/g was obtained. The content of amine nitrogen was 0.6%, crude protein – 12,8%, ODS – 18,0%.The presence of 13 major protein fractions was established by electrophoretic studies of fractional composition of CFP protein substances. The results of identification of peptide fractions showed that more than 50% of bioactive peptides with molecular weight (MM) from 15 to 40 kDa showed proteolytic activity. Peptide fractions WITH mm 57-60 kDa were characterized by the presence of β-glucanase activity. 4 peptide fractions with MM 20,9; 26,7; 35,0 and 51,4kDa showed amylolytic activity. The results of the residual biomass study showed that it contained 20,8% crude protein, 31,0% polysaccharides (ODS), 3,3% reducing carbohydrates, 0,4% amine nitrogen and 1,6% amino acids in free form. According to the content of essential amino acids in the protein, the fungal biomass is not inferior to animal proteins, is close to the FAO/who scale and is superior to such amino acids as methionine, tryptophan (10,2 against 3,5 and 18,0 against 1,0 g/100g of protein, respectively), yielding to such amino acids as valine and leucine (3,4 against 5,0 and 4,3 against 7,0 g/100g of protein, respectively). The presence of a high amount of tryptophan and methionine in microbial biomass opens up prospects for the use of the latter to create dietary SUPPLEMENTS for functional purposes. 

KeywordsAspergillus oryzae, enzymes, fungal biomass, fermentoliz, functional ingredients
AcknowledgmentThe studies were carried out at the expense of subsidies for the implementation of the state task within Programs of Basic Scientific Research of the State Academies of Sciences for 2013-2020. (topic number 0529-2015-0108).
Received27.10.2018
Publication date29.10.2018
Number of characters2273
Cite   Download pdf To download PDF you should sign in
Размещенный ниже текст является ознакомительной версией и может не соответствовать печатной

views: 1183

Readers community rating: votes 0

1. Golubev, V.N. Pischevaya biotekhnologiya / V.N. Golubev, I.N. Zhiganov//-M.:DeLiprint, 2001.- 123 s.

2. Nguen Kuok Nguen. Shtamm mikromitseta Aspergillusoryzae – produtsent proteoliticheskikh i amiloliticheskikh fermentov dlya ispol'zovaniya v pischevoj promyshlennostit, patent №2499039 OOO «Sostra», 20.11.2013., byul.32

3. Polyakov, V.A. Instruktsiya po tekhno-khimicheskomu i mikrobiologicheskomu kontrolyu spirtovogo proizvodstva / V.A. Polyakov i dr.// M.: DeLiprint, - 2007. - 480 s.

4. Rimareva, L.V. Biotekhnologicheskie aspekty sozdaniya pischevykh dobavok biokorregiruyuschego dejstviya na osnove mikrobnoj biomassy / L.V. Rimareva, E.I. Kurbatova, N.A. Fursova i dr.// Khranenie i pererabotka sel'khozsyr'ya. - 2011. - № 2. - S. 45-47.

5. Serba, E.M. Skrining aktivnykh populyatsij griba Aspergillusoryzae po sposobnosti k sintezu promyshlenno znachimykh metabolitov / E.M. Serba, M.B. Overchenko, L.V. Rimareva i dr.// Mikologiya i fitopatologiya. – 2017. - № 1. – S.34-40.

6. Serba, E.M. Mitselial'nye griby – perspektivnyj istochnik gidrolaz i tsennykh biopolimerov / E.M. Serba, L.V. Rimareva, M.B. Overchenko i dr. // Vestnik rossijskoj sel'skokhozyajstvennoj nauki. - 2016. - № 4. - S. 41-43.

7. Serba, E.M. Razrabotka metoda opredeleniya soderzhaniya polisakharidov v biomassakh mikroorganizmov / E.M. Serba, M.B. Overchenko, A. Yu. Krivova i dr. // Khranenie i pererabotka sel'khozsyr'ya. – 2015- № 7.- S. 32-35.

8. Shenderov, B.A. Funktsional'noe pitanie i ego rol' v profilaktike metabolicheskogo sindroma / Monografiya. – M.: DeLiprint, 2008. – 320 s.

9. New, N. Characterization of chitosan-glucan complex extracted from the cell wall of fungus Gongronellabutleri USDB 0201 by an enzymatic method / N. New, W.F. Stevens , S. Tokura, H.Tamura // Enzyme and Microbial Technology. – 2008. - V. 42. – R. 242–251.

10. Ward, O.P. Physiology and biotechnology of Aspergillus /O.P. Ward,W. M. Qin, N.J. Dhanjoon, J.Ye, A. Singh // Adv Appl. Microbiol. – 2006. – V. 58. - № 1.- P.75.

Система Orphus

Loading...
Up