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Polymorphisms of the promote region of the TNFA gene and their two-locus haplotypes in patients with ulcerative colitis russian population of the Chelyabinsk region
 
PIIS102872210002425-2-1
DOI10.31857/S102872210002425-2
Publication type Article
Status Published
Authors
D. Stashkevich 
Affiliation: Chelyabinsk State University
A. Kobelyatskaya
Affiliation: Chelyabinsk State University
A. Burmistrova
Affiliation: Chelyabinsk State University
Journal nameRossiiskii immunologicheskii zhurnal
EditionVolume 12 Issue 3
Pages444-448
Abstract

The results of the study on the distribution of frequency of occurrence of allelic variants, genotypes and haplotypes of polymorphisms of the gene TNFA-1031T/C, –863C/A, –308G/A, –238G/A in patients with nonspecifi c ulcerative colitis of the Russian population of the Chelyabinsk region are presented. Genotyping of the TNFA promoter region was carried out by polymorphism of restriction fragment lengths followed by detection by horizontal electrophoresis in 3% agarose gel. In the group of patients with UC, an increase in the frequency of occurrence of the TNFA-238*A allele, the heterozygous genotype of TNFA-238GA, the homozygous genotype from the ancestral allele –863CC, the two-locus haplotypes TNFA-863*CTNFA- 238*A, TNFA-308*G-TNFA-238*A, TNFA-238*A-TNFA-1031*C was found. In the group of patients carriers of heterozygous genotype TNFA-863C/A, homozygous genotype TNFA-238GG, two-locus haplotypes TNFA-308*G-TNFA-238*G, TNFA-238*G-TNFA-1031*C were less common. Inherited models for the following polymorphic sites of the TNFA gene have been identifi ed: a superdominant model is characteristic of TNFA-863C/A, and codominant – for TNFA-238G/A. The obtained data indicate that polymorphism –238G/A TNFA is signifi cant for the for development of predisposition to ulcerative colitis.

Keywordsgenetic polymorphism, tumor necrosis factor alpha, ulcerative colitis
Received12.01.2019
Publication date12.01.2019
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1. Glavnov P. V., Lebedeva N. N., Kaschenko V. A., Varzin S. A. Yazvennyj kolit i bolezn' Krona. Sovremennoe sostoyanie problemy ehtiologii, rannej diagnostiki i lecheniya. Vestnik SPbGU 2015, 4, 48–72. [Glavnov P. V., Lebedeva N. N., Kash’enko V.A., Varzin C. A. Ulcerative colitis and Crohn disease. The modern state of problem of etiology, early diagnostics and treatment. Vestnik SPbGU2015, 4, 48–72].
2. Konovich E. A., Khalif I. L., Shapina M. V. Immunopatogenez vospalitel'nykh zabolevanij kishechnika. Rossijskij zhurnal gastroehnterologii, gepatologii, koloproktologii 2013, 4, 69–78.[Konovich E. A., Xalif I. L., Shapina M. V. Immunopatogenesis of irritable bowel diseases. Russian journal of immunology 2013, 4, 69–78].
3. Gok I., Ucar F., Ozgur O. Infl ammatory cytokine gene polymorphism profi les in Turkish patients with ulcerative colitis. Medicinski Glasnik 2015, 12 (1), 33–39.
4. Ketlinskij S. A., Simbirtsev A. S. Tsitokiny: SPb.: Foliant, 2008, 552 s. [Ketlinskiy S. A., Simbirtsev A. S. Cytokine. St. Petersburg. Foliant, 2008, 552].
5. Stashkevich D. S., Burmistrova A. L., Kobelyatskaya A. A. Polimorfizm genov provospalitel'nykh tsitokinov IL-1b, IL-6, TNFA u bol'nykh nespetsificheskim yazvennym kolitom russkoj populyatsii Chelyabinskoj oblasti. Sovremennye problemy nauki i obrazovaniya. 2016, 4, 1–8. [Ehlektronnyj resurs] (URL: https://science-education.ru/ru/article/  view?id=25083) [Stashkevich D. S., Burmistrova A. L., Kobelyatskaya A. A. Polymorphism of the genes of the proinfl ammatory cytokines IL-1b, IL-6, TNFA in patients with ulcerative colitis of the Russian population of the Chelyabinsk region. Modern problems of science and education. 2016, 4, 1–8. [An electronic resource]].
6. Stashkevich D. S. Alleli i genotipy genov osnovnykh tsitokinov i ikh mezhgennye i vnutrigennye svyazi v assotsiatsii s revmatoidnym artritom u russkoj i bashkirskoj populyatsij.: avtoref dis. … kand. biol. nauk: 14.00.36 Chelyabinsk., 2009. 23 s. [Stashkevich D. S. Alleles and genotypes of genes of the main cytokines and their intergenic and intragenic relationships in association with rheumatoid arthritis in Russian and Bashkir populations. … cand. Biol. Sciences: 14.00.36 Chelyabinsk., 2009. 23 p.].
7. TNF tumor necrosis factor [Homo sapiens (human)] [Electronic resource] / 11-Apr-2018. (URL: https:// www.ncbi.nlm.nih.gov/gene/7124).
8. SNPStats: Your web tool for SNP analysis [Electronic resource] / Institut Catala d’Oncologia, 2016. (URL: https://www.snpstats.net/start.htm?).

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