CD57 expression by peripheral blood cytotoxic T cells in sarcoidosis

 
PIIS102872210002405-0-1
DOI10.31857/S102872210002405-0
Publication type Article
Status Published
Authors
Affiliation:
Academician I. P. Pavlov First St. Petersburg State Medical University
Institute of Experimental Medicine
Affiliation: Academician I.P. Pavlov First St. Petersburg State Medical University
Affiliation: Academician I.P. Pavlov First St. Petersburg State Medical University
Affiliation: Institute of Experimental Medicine
Affiliation:
Academician I.P. Pavlov First St. Petersburg State Medical University
State Research Institute of Highly Pure Biopreparations
Affiliation: Academician I.P. Pavlov First St. Petersburg State Medical University
Affiliation:
Academician I.P. Pavlov First St. Petersburg State Medical University
St. Petersburg Pasteur Institute,
Journal nameRossiiskii immunologicheskii zhurnal
EditionVolume 12 Issue 3
Pages329-334
Abstract

Using 10-color fl ow cytometry we characterized cytotoxic T cell subsets based on expression of CD45RA, CD62L, CD27, and CD28 and compared the expression of CD57 between these subsets in peripheral blood sample from patients with chronic sarcoidosis (n=46) and healthy volunteers (n=46). Circulating naive, central and eff ector memory as well as TEMRA CD3+CD8+ cells of sarcoidosis patients contained signifi cantly increased proportions CD57+ cells when compared with healthy controls. Interestingly, CD57 expression was increased in subsets with ‘non mature’ and intermediate phenotypes – EM1, EM2, EM4, pE1 and pE2. Our results indicate the involvement of CD3+CD8+ in maintenance in sarcoidosis as well as suggest CD57-positive cells to be the novel target for evaluation the eff ectiveness of sarcoidosis therapy.

Keywordssarcoidosis, cytotoxic T cells, CD57 expression, fl ow cytometry
Received11.01.2019
Publication date12.01.2019
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