Calorimetric measurements of heat production in plant cell mitochondria

Measurements of heat production in the plant cell mitochondria were performed on the experimental sample of the nanocalorimeter. The addition was carried out without delay and with a delay of 1.5 hours at a tempera-ture of 24 °C. The results of measurements of heat production in freshly isolated mitochondria of winter wheat in a suspension medium showed a 6-fold decrease in heat production in the case of a delayed experiment, which confirmed the need for measurements without delay in time to ensure precise measurements.

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08.10.2018

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10.10.2018

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528

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GOST	Moiseeva S., Voinikov V., Kotelnikov G., Pobezhimova , Grabelnykh O. Calorimetric measurements of heat production in plant cell mitochondria // Nauchnoe priborostroenie – 2018. – V. 28. – Issue 3 C. 59-62 [Electronic resource]. URL: http://ras.jes.su/np/s207987840000385-2-1-en (circulation date: 26.04.2024). DOI: 10.31857/S086858860001097-8
MLA	Moiseeva, Sof'ya, Voinikov, Viktor, Kotelnikov, Grigory, Pobezhimova, T., Grabelnykh, Olga "Calorimetric measurements of heat production in plant cell mitochondria." Nauchnoe priborostroenie 28.3 (2018):59-62. DOI: 10.31857/S086858860001097-8
APA	Moiseeva S., Voinikov V., Kotelnikov G., Pobezhimova , Grabelnykh O. (2018). Calorimetric measurements of heat production in plant cell mitochondria. Nauchnoe priborostroenie 28(3), pp.59-62 DOI: 10.31857/S086858860001097-8

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1. Nakamura T., Matsuoka I. Calorimetric studies of heat of respiration of mitochondria. Journal of Biochemistry, 1978, vol. 84, no. 1, pp. 39–46.

2. Zhou P.-J., Zhou H.-T., Liu Y., Qu S.-S., Zhu Y.-G., Wu Z.-B. Studies on the energy release of rice mitochon-dria under different conditions by means of microcalori-metry. Journal of Biochemical and Biophysical Methods, 2001, vol. 48, pp. 1–11.

3. Velázquez-Campoy A., López-Mayorga O., Cabrerizo-Vı́ lchez M.A. Development of an isothermal titration mi-crocalorimetric system with digital control and dynamic power Peltier compensation. I. Description and basic per-formance. Review of Scientific Instruments, 2000, vol. 71, no. 4, pp. 1824–1831. Doi: 10.1063/1.1150543.

4. Garcia-Fuentes L., Baron C., Mayorga O.L. Influence of dynamic power compensation in an isothermal titration microcalorimeter. Analytical Chemistry, 1998, vol. 70, no. 21, pp. 4615–4623. Doi: 10.1021/ac980203u.

5. Wiseman Т., Williston S., Brandts J.F., Lin L.-N. Rapid measurement of binding constants and heats of binding using a new titration calorimeter. Analytical Biochemistry, 1989, vol. 179, no. 1, pp. 131–137.

6. Kotelnikov G.V., Moiseyeva S.P., Mezhburd E.V., Krayev V.P. New isothermal titration calorimeter for in-vestigationson very small samples. Theoretical and expe-rimental studies. Journal of Thermal Analysis and Calo-rimetry, 2002, vol. 68, no. 3, pp. 803–818.

7. Kotelnikov G.V., Moiseyeva S.P., Mezhburd E.V. Maevsky E.I., Grishina E.V. Studying dispersoid systems method of introducing an injecting needle into calorime-tric chamber of capillary titration calorimeter. Journal of Thermal Analysis and Calorimetry, 2005, vol. 81, no. 2, pp. 255–259. Doi: 10.1007/s10973-005-0775-6.

8. Kotelnikov G.V., Moiseyeva S.P., Mezhburd E.V., Krayev V.P. Method of separating the sensitive volume of calorimetric cells in a differential titration calorimeter. Journal of Thermal Analysis and Calorimetry, 2000, vol. 62, no. 1, pp. 39–50. Doi: 10.1023/A:1010150409126.

9. Pobezhimova T.P., Kolesnichenko A.V., Grabel'nyh O.I. Metody izucheniya mitohondrij rastenij. Polyarografiya i ehlektroforez [Methods of studying of mitochondrions of plants. Polyarografiya and electrophoresis]. Moscow, OOO NPK Promehkspobezopasnost Publ., 2004. 98 p. (In Russ.).

10. Kotelnikov G.V., Moiseyeva S.P. [Nanocalorimeter for measuring the heat production in mitochondria]. Nauch-noe Priborostroenie [Scientific Instrumentation], 2016, vol. 26, no. 3, pp. 3–9. Doi: 10.18358/np-26-3-i39. (In Russ.).