Polyprenyl phosphates induce a high humoral and cellular response to immunization with recombinant proteins of the replicative complex of the hepatitis C virus

 
PIIS086956520003094-6-1
DOI10.31857/S086956520003094-6
Publication type Article
Status Published
Authors
 
Affiliation: N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology
Address: Russian Federation, Moscow
Affiliation: N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology
Address: Russian Federation
Affiliation: Peoples Friendship University of Russia
Address: Russian Federation
Affiliation: N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology
Address: ,
Affiliation: N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology
Address: Russian Federation
Affiliation: N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology
Address: Russian Federation
Affiliation: N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology
Address: Russian Federation
Affiliation: N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology
Address: Russian Federation
Affiliation: N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology
Address: Russian Federation
Affiliation: N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology
Address: Russian Federation
Journal nameDoklady Akademii nauk
EditionVolume 482 Issue 4
Pages459-462
Abstract

The search for new adjuvants remains the critical task for the creation of hepatitis C vaccines due to the weak immunogenicity of biotechnological products. When immunizing mice with the recombinant proteins NS3 and NS5B of the hepatitis C virus (HCV), the adjuvant activity of three immunomodulators was compared. Phosprenyl® on the basis of polyprenyl phosphate (PPP), chemically synthesized analogue of the bacterial cell wall glucosaminyl muramyl dipeptide (GMDP), and IFN-α recombinant protein were tested. GMDP increased the activity of IgG1 antibodies in 4-6 times, but did not stimulate the production of IFN-γ; IFN-α has not shown any adjuvant properties. The introduction of recombinant HCV proteins together with PPP in low doses increased the activity of IgG2a isotype antibodies in 4-7 times and increased of IFN-γ secretion 3-fold. Thus, it was first shown that the PPP polarizes the immune response to Th1- type and is a promising adjuvant for the development of a vaccine against hepatitis C.

Keywordshepatitis C virus, polyprenyl phosphates, immune response, adjuvants
Received10.11.2018
Publication date10.11.2018
Cite   Download pdf To download PDF you should sign in
Размещенный ниже текст является ознакомительной версией и может не соответствовать печатной

views: 1482

Readers community rating: votes 0

1. Walker C.M. // Curr. Opin. Virol. 2017. V. 23. P. 113–119.

2. Naarding M.A., Falkowska E., Xiao H., Dragic T. // Vaccine. 2011. V. 29. № 16. P. 2910–2917.

3. Mukovnya A.V., Tunitskaya V.L., Khandazhinskaya A.L., Golubeva N.A., Zakirova N.F., Ivanov A.V., Kukhanova M.K., Kochetkov S.N. // Biokhimiya. 2008. T. 73. № 6. S. 822–832.

4. Ivanov A.V., Korovina A.N., Tunitskaya V.L., Kostyuk D.A, Rechinsky V.O, Kukhanova M.K., Kochetkov S.N. // Protein Expr. Purif. 2006. V. 48, No. 1. P. 14–23.

5. Masalova O.V., Lesnova E.I., Grabovetskij V.V., Smirnova O.A., Ulanova T.I., Burkov A.N., Ivanov A.V., Zaberezhnyj A.D., Ataullakhanov R.I., Kusch A.A. // Molekulyarnaya biologiya. 2010. T. 44, № 2. S. 275–283.

6. Ershov F.I. Sistema interferona v norme i pri patologii. M.: Meditsina, 1996, 239 s.

7. Masalova O.V., Lesnova E.I., Shingarova L.N., Tunitskaya V.L., Ulanova T.I., Burkov A.N., Kusch A.A. // Molekulyarnaya biologiya. 2012. T. 46. № 3. S. 525–534.

8. Pronin A.V., Grigorieva E.A., Sanin A.V., Narovlyansky A.N., Ozherelkov S.V., Deyeva A.V., Danilov L.L., Maltsev S.D., Najid A. // Rossijskij immunologicheskij zhurnal. 2002. T. 7. № 2. P. 135–142.

9. Pronin A.V., Narovlyansky A.N., Shulzhenko A.E., Sanin A.V., Sedov A.M. // Cytokine Growth Factor Rev. 2016. V. 30. P. 119–126.

10. Ozherelkov S.V., Sanin A.V., Narovlyanskij A.N., Pronin A.V., Kozhevnikova T.N. // Vetkorm. 2017. № 3. S. 81–83.

11. Chmielewska A.M., Naddeo M., Capone S., et al. // J. Virol. 2014. V. 88. № 10. P. 5502–5510.

12. Jin J., Yang J.Y., Liu J., et al. // World J. Gastroenterol. 2002. V. 8. № 3. P. 505–510.

Система Orphus

Loading...
Up