Polyprenyl phosphates induce a high humoral and cellular response to immunization with recombinant proteins of the replicative complex of the hepatitis C virus

 
PIIS086956520003094-6-1
DOI10.31857/S086956520003094-6
Publication type Article
Status Published
Authors
 
Affiliation: N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology
Address: Russian Federation, Moscow
Affiliation: N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology
Address: Russian Federation
Affiliation: Peoples Friendship University of Russia
Address: Russian Federation
Affiliation: N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology
Address: ,
Affiliation: N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology
Address: Russian Federation
Affiliation: N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology
Address: Russian Federation
Affiliation: N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology
Address: Russian Federation
Affiliation: N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology
Address: Russian Federation
Affiliation: N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology
Address: Russian Federation
Affiliation: N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology
Address: Russian Federation
Journal nameDoklady Akademii nauk
EditionVolume 482 Issue 4
Pages459-462
Abstract

The search for new adjuvants remains the critical task for the creation of hepatitis C vaccines due to the weak immunogenicity of biotechnological products. When immunizing mice with the recombinant proteins NS3 and NS5B of the hepatitis C virus (HCV), the adjuvant activity of three immunomodulators was compared. Phosprenyl® on the basis of polyprenyl phosphate (PPP), chemically synthesized analogue of the bacterial cell wall glucosaminyl muramyl dipeptide (GMDP), and IFN-α recombinant protein were tested. GMDP increased the activity of IgG1 antibodies in 4-6 times, but did not stimulate the production of IFN-γ; IFN-α has not shown any adjuvant properties. The introduction of recombinant HCV proteins together with PPP in low doses increased the activity of IgG2a isotype antibodies in 4-7 times and increased of IFN-γ secretion 3-fold. Thus, it was first shown that the PPP polarizes the immune response to Th1- type and is a promising adjuvant for the development of a vaccine against hepatitis C.

Keywordshepatitis C virus, polyprenyl phosphates, immune response, adjuvants
Received10.11.2018
Publication date10.11.2018
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