Membrane-bound Bacteriophytochrome-like Complex of Phototrophic Purple Non-sulfur Bacterium Rhodopseudomonas Palustris

Publication type Article
Status Published
Affiliation: Institute of Basic Biological Problems, RAS
Address: Russian Federation, Pushchino
Affiliation: Institute of Basic Biological Problems, RAS
Address: Russian Federation, Pushchino
Affiliation: Institute of Basic Biological Problems, RAS
Address: Russian Federation, Pushchino
Journal nameDoklady Akademii nauk
EditionVolume 482 Issue 6

A pigment-protein complex of yellow color with absorption maxima at 682 nm and 776 nm, characteristic for bacteriophytochromes, was isolated from the photosynthetic membranes of the purple bacterium Rhodopseudomonas palustris. Zinc-induced fluorescence of the complex indicated the presence of the biliverdin chromophore covalently bound to the protein. The parameters of low-temperature fluorescence (λ excitation at 680 nm, λ emission at 695 nm) indicated the complex's ability to photoconversion. These data, as well as the kinetics of accumulation of the red (Pr) -form on far red light, allowed the complex to be classified as a bacteriophytochrome-like complex with its localization in photosynthetic membranes Rps. palustris.

Publication date13.12.2018
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