Comparative evaluation cytokine-like activity of Staphylococcus aureus by methods multiplex and ELISA

 
PIIS102872210002428-5-1
DOI10.31857/S102872210002428-5
Publication type Article
Status Published
Authors
Affiliation: Institute of Immunology and Physiology UrB RAS
Affiliation: Institute of Immunology and Physiology UrB RAS
Affiliation:
Institute of Immunology and Physiology UrB RAS
South-Ural State University (National Research University)
Affiliation: Institute of Immunology and Physiology UrB RAS
Affiliation: State research Institute of high-purity biological products
Affiliation: Institute of Cellular and Intracellular Symbiosis UrB RAS
Journal nameRossiiskii immunologicheskii zhurnal
EditionVolume 12 Issue 3
Pages460-465
Abstract

The paper presents the results of investigations of supernatants of the daily broth cultures of Staphylococcus aureus to the defi nition of cytokine-like substances (INF-γ, INF-α, IL-1Ra, IL-1, IL-4, IL-6, IL-8, IL-10, IL-17a, TNF-α) by the method of enzyme immunoassay with the use of the “Cytokine” kits (St. Petersburg), as well as multiplex analysis using the BioRad immunofl uorescence test-systems (USA) and Multiplex (Luminex, USA). It was found that cytokine-like substances (IL-1Ra, IL-1β, TNFalfa and INF-γ) were detected in supernatants of some strains of Staphylococcus aureus by both methods (immunoenzyme and immunofl uorescent), whereas IL-10, IL-6, IL-8 and IL-17a were determined only by immunofl uorescent method, but INF-α – only by enzyme immunoassay. It was noted that a part of the cytokine-like substances in a greater concentration were determined by the method of immunofl uorescence analysis (IL-1Ra, TNF-alfa), and also as INF-γ and IL-4 by the method of enzyme immunoassay.

Keywordscytokines, S. aureus, cytokine-like substances
AcknowledgmentThe work was done on the topic from the Plan of Research and Development IIF UB RAS, № state. the registration of AAAA-A18–118020690020–1, and the topic from the Plan of R & D of ICWS, UB RAS, No. of State. registration number 116021510075.
Received12.01.2019
Publication date12.01.2019
Cite   Download pdf To download PDF you should sign in
Размещенный ниже текст является ознакомительной версией и может не соответствовать печатной

views: 1239

Readers community rating: votes 0

1. Zurochka A. V., Dukardt V. V., Zurochka V. A., Dobrynina M. A., Zueva E. B., Tyapaeva Ya. V., Gritsenko V. A. Bakterii kak produtsenty tsitokinopodobnykh veschestv. Rossijskij immunologicheskij zhurnal 2017, 11 (20), 3, 374–376.

2. Zurochka A. V., Zurochka V. A., Dobrynina M. A., Zueva E. B., Tyapaeva Ya. V., Dukardt V. V., Gritsenko V. A. Tsitokinovaya i antitsitokinovaya aktivnost' stafilokokkov. Metodicheskie osobennosti. Rossijskij immunologicheskij zhurnal 2017, 11 (20), 4, 707–709.

3. Zurochka A. V., Dukardt V. V., Zurochka V. A., Dobrynina M. A., Zueva E. B., Tyapaeva Ya. V., Gritsenko V. A. Stafilokokki kak produtsenty tsitokinopodobnykh veschestv. Rossijskij immunologicheskij zhurnal. 2017, 11 (20), 2, 134–136.

4. Zurochka A. V., Dukardt V. V., Zurochka V. A., Dobrynina M. A., Zueva E. B., Belozertseva Yu. P., Tyapaeva Ya. V., Gritsenko V. A. Staphylococcus aureus: spontannaya produktsiya tsitokino-podobnykh veschestv i eyo regulyatsiya sinteticheskim analogom aktivnogo tsentra granulotsitarno-makrofagal'nogo koloniestimuliruyuschego faktora (GM-KSF). Byulleten' Orenburgskogo nauchnogo tsentra UrO RAN. 2017, 1, 15 c. [Ehlektr. resurs] (URL: http://elmag.uran.ru:9673/magazine/ Numbers/2017–1/Articles/ZAV-2017–1.pdf).

5. Zurochka A. V., Zurochka V. A., Zueva E. B., Dobrynina M. A., Dukardt V. V., Yu. P. Belozertseva, Tyapaeva Ya. V., Gritsenko V. A. Sravnitel'naya otsenka vliyaniya sinteticheskogo peptida aktivnogo tsentra GM-KSF (Zp2) i supernatantov sutochnykh kul'tur gramotritsatel'nykh i grampolozhitel'nykh bakterij na produktsiyu tsitokinov nejtrofilami perifericheskoj krovi cheloveka. Rossijskij immunologicheskij zhurnal 2016, 10 (19), 4, 430– 433.

6. Javed N., Xue G., Lu A., Xing Y., Iwakura Y., Xiao H., Lecoeur H., Späth G. F., Meng G. Cross reactivity of S. aureus to murine cytokine assays: A source of discrepancy. Cytokine 2016, 81, 101–108.

7. Medik V.A., Tokmachev M. S., Fishman B. B. Statistika v meditsine i biologii. «Teoreticheskaya statistika». Pod red. Komarova Yu. M.. Meditsina, Moskva, 2000, 1, 454.

Система Orphus

Loading...
Up