Comparative evaluation cytokine-like activity of Staphylococcus aureus by methods multiplex and ELISA

The paper presents the results of investigations of supernatants of the daily broth cultures of Staphylococcus aureus to the defi nition of cytokine-like substances (INF-γ, INF-α, IL-1Ra, IL-1, IL-4, IL-6, IL-8, IL-10, IL-17a, TNF-α) by the method of enzyme immunoassay with the use of the “Cytokine” kits (St. Petersburg), as well as multiplex analysis using the BioRad immunofl uorescence test-systems (USA) and Multiplex (Luminex, USA). It was found that cytokine-like substances (IL-1Ra, IL-1β, TNFalfa and INF-γ) were detected in supernatants of some strains of Staphylococcus aureus by both methods (immunoenzyme and immunofl uorescent), whereas IL-10, IL-6, IL-8 and IL-17a were determined only by immunofl uorescent method, but INF-α – only by enzyme immunoassay. It was noted that a part of the cytokine-like substances in a greater concentration were determined by the method of immunofl uorescence analysis (IL-1Ra, TNF-alfa), and also as INF-γ and IL-4 by the method of enzyme immunoassay.

Keywords

cytokines, S. aureus, cytokine-like substances

Acknowledgment

The work was done on the topic from the Plan of Research and Development IIF UB RAS, № state. the registration of AAAA-A18–118020690020–1, and the topic from the Plan of R & D of ICWS, UB RAS, No. of State. registration number 116021510075.

Received

12.01.2019

Publication date

12.01.2019

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GOST	Fomina L., Fayzullina A., Zurochka V., Dobrynina M., Symbircev A., Gritsenko V. Comparative evaluation cytokine-like activity of Staphylococcus aureus by methods multiplex and ELISA // Rossiiskii immunologicheskii zhurnal – 2018. – V. 12. – Issue 3 C. 460-465 [Electronic resource]. URL: http://ras.jes.su/ruimm/s207987840001622-3-1-en (circulation date: 25.04.2024). DOI: 10.31857/S102872210002428-5
MLA	Fomina, L., Fayzullina, A., Zurochka, V., Dobrynina, M., Symbircev, A., Gritsenko, V. "Comparative evaluation cytokine-like activity of Staphylococcus aureus by methods multiplex and ELISA." Rossiiskii immunologicheskii zhurnal 12.3 (2018):460-465. DOI: 10.31857/S102872210002428-5
APA	Fomina L., Fayzullina A., Zurochka V., Dobrynina M., Symbircev A., Gritsenko V. (2018). Comparative evaluation cytokine-like activity of Staphylococcus aureus by methods multiplex and ELISA. Rossiiskii immunologicheskii zhurnal 12(3), pp.460-465 DOI: 10.31857/S102872210002428-5

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