The role of human chorionic gonadotropin and its peptide fragments in the regulation of IDO expression by human monocytes

 
PIIS102872210002400-5-1
DOI10.31857/S102872210002400-5
Publication type Article
Status Published
Authors
Affiliation: Institute of Ecology and Genetics of Microorganisms UB RAS
Affiliation: Institute of Ecology and Genetics of Microorganisms UB RAS
Affiliation: Institute of Ecology and Genetics of Microorganisms UB RAS
Affiliation: Institute of Ecology and Genetics of Microorganisms UB RAS
Affiliation: Institute of Ecology and Genetics of Microorganisms UB RAS
Journal nameRossiiskii immunologicheskii zhurnal
EditionVolume 12 Issue 3
Pages306-310
Abstract

The role of human chorionic gonadotropin (hCG) and its peptide fragments of β-subunit (LQGV, AQGV, VLPALP) in the regulation of indolеamine-2,3-dioxygenase (IDO) expression in human monocytes was studied in vitro. It is elucidated, that a high concentration of hCG (20 μg/ml) had an independent stimulatory eff ect on the expression of IDO in LPS -, and the IFN-γ – induced monocytes. Peptides AQGVand LQGV (20 μg/ml) also stimulated LPS-induced expression of IDO, but had no eff ect on IFN- γ-induced expression of the enzyme. At the same time, peptide VLPALP (20 μg/ml) provided the stimulatory eff ect only on the IFN-γ-induced expression of IDO. Simultaneously, peptides AQGV and VLPALP increased the production of IL-1β monocytes under LPS-stimulation, while hCG did not exert any independent eff ect. Generally, hCG and oligopeptides of β-hCG subunit enhance the expression of IDO by monocytes, which eventually contributes to peripheral immune tolerance development.

Keywordschorionic gonadotropin, regulatory peptides (LQGV, AQGV, VLPALP), indoleamine-2,3- dioxygenase (IDO), immune tolerance, monocytes
AcknowledgmentThe work was performed in the framework of the state assignment, N state registration topics: 01201353248
Received11.01.2019
Publication date12.01.2019
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1. Mellor A. L., Munn D. H. Tryptophan catabolism prevents maternal T-cell from activating lethal anti-fetal immune responses // J. Reprod. Immunol. 2001. 52(1Ts2), 5Ts13.

2. Koldehoff M., Cierna B., Steckel N. K., Beelen D. W., Elmaagacli A. H. Maternal molecular features and gene profi ling of monocytes during fi rst trimester pregnancy // J. Reprod. Immunol. 2013. 99 (1Ts2), 62Ts68.

3. Fallarino F., Grohmann U., Vacca C., Bianchi R., Orabona C., Spreca A., Fioretti M. C., Puccetti P. T-cell apoptosis by tryptophan catabolism // Cell. Death Diff er. 2002. 9(10), 1069Ts1077.

4. Schumacher A. Human chorionic gonadotropin as a pivotal endocrine immune regulator initiating and preserving tolerance // International Journal of Molecular Science. 2017. 18, 2166.

5. Elmaagacli A. H., Ditschkowski M., Steckel N. K., Gromke T., Ottinger H., Hillen U., Baba H. A., Trenschel R., Beelen D. W., Koldehoff M. Human chorionic gonadotropin and indolamine 2,3-dioxygenase in patients with GVHD // Bone Marrow Transplant. 2014. 6(49), 800Ts805.

6. Khan N. A., Benner R. Human chorionic gonadotropin: a model molecule foroligopeptide-based drug discovery // Endocr. Metab. Immune Disord. Drug Targets. 2011. 1(11), 32Ts53.

7. Van der Zee M., Dik W. A., Kap Y. S., Dillon M. J. Synthetic human chorionic gonadotropin-related oligopeptides impair early innate immune responses to Listeria monocytogenes in Mice // J. Infect. Dis. 2010. 201(7), 1072Ts1080.

8. «amorina —. ј., Ўirshev —. ¬. ќligopeptidy ?-sub'edinitsy khorionicheskogo gonadotropina v induktsii differentsirovki “-kle tok v Treg i Th17 // Ѕyulleten' ehksperimental'noj biologii i meditsiny. 2015. T. 160. є 7. —. 84Ts87.[Zamorina S. A., Shirshev S. V. Oligopeptides of chorionic gonadotropin ?-subunit in induction of T cell diff erentiation into Treg and Th17 // Bulletin of Experimental Biology and Medicine. 2015. 160. (7), 84Ts87. Russian.]

9. Cole L. A. HCG, the wonder of todayTs science // Reprod. Biol. Endocrinol. 2012. 10, 24.

10. Landmann R. L.C., Obrist R., Obrecht J. P. Eff ect of cytokines and lipopolysaccharide on CD14 antigen expression in human monocytes and macrophages // J. Cell. Biochem. 1991. 47 (4), 317Ts329.

11. Jung I. D., Jung D., Lee C. M., Jeong J. M., Lee J. S., Park W. S., Han J., Park Y. M. Diff erential regula-tion of indoleamine 2,3-dioxygenase by lipopolysaccharide and interferon gamma in murine bone marrow derived dendritic cells // FEBS Lett. 2007. 7(581), 1449Ts 1456.

12. Babu M. M., van der Lee R., Sanchez de Groot N., Gsponer J. Intrinsically disordered proteins: regulation and disease // Curr. Opin. Struct. Biol. 2011. 21(3), 432Ts440.

13. Tompa P., Davey N. E., Gibson T. J., Babu M. A million peptide motifs for the molecular biologist // Mol. Cell. 2014. 55(2), 161Ts169.

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