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Protein Trap: How do Green Chimeras «Catch» Genes
 
PIIS0032874X0001084-1-1
DOI10.31857/S0032874X0000479-5
Publication type Article
Status Published
Authors
S. Fedorova 
Affiliation:
Novosibirsk National Research State University
Institute of Cytology and Genetics, Siberian Branch, RAS
Address: Russian Federation, Novosibirsk
A. Kruscheva
Affiliation: Novosibirsk National Research State University
Address: Russian Federation
N. Dorogova
Affiliation: Institute of Cytology and Genetics, Siberian Branch, RAS
Address: Russian Federation
Journal namePriroda
EditionIssue №8
Pages10-17
Abstract

Protein trap is one of the modern genetic techniques, based on introducing of green fluorescent protein (GFP) coding sequence into the genome. The modern research tools developed on a protein trap basis allow artificially altering the intracellular amount of hybrid RNAs or proteins. Here we give examples of various methods of using the protein traps — from the analysis of the specific genes expression and the subcellular localization of the inverstigated pro teins to the studies of interactions between different organism’s tissues or organs.

KeywordsGFP, green fluorescent protein, recombinant proteins, gene expression, intracellular markers
Received20.09.2018
Publication date04.10.2018
Number of characters527
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