Mass-spectrometric study of unithiol water-acetonitril solutions

 
PIIS086858860001099-0-1
DOI10.31857/S086858860001099-0
Publication type Article
Status Published
Authors
Affiliation: Institute for Analytical Instrumentation of RAS
Address: Russian Federation, Saint-Petersburg
Affiliation: Institute for Analytical Instrumentation of RAS
Address: Russian Federation
Affiliation: Institute for Analytical Instrumentation of RAS, Saint-Petersburg
Address: Russian Federation
Affiliation: Institute for Analytical Instrumentation of RAS
Address: Russian Federation
Affiliation: Ioffe Institute
Address: Russian Federation
Affiliation: Institute of Cytology RAS
Address: Russian Federation
Journal nameNauchnoe priborostroenie
EditionVolume 28 Issue 3
Pages72-83
Abstract

Unithiol test is widely used for study of biological effect on living organisms of geomagnetic and space physical factors; the test is based on semi oxidation time measurements done electrochemically or photo metrically. To measure the products of unithiol oxidation reaction and rates of their production, ESI massspectrometric study has been made. Unithiol associates cathionated with Na+ have been found in mass-spectra of its water-acetonitrile solutions with general formula (C3H7O3S3Na)nNa+ with n up to 8; they present in essential amount together with Na+ cathionated monomer ion. Associate peak intensities were decreased with the molecular number, but their total intensity was higher than that of monomer. The associate total intensity grows with increase in concentration, this is an evidence for their production directly in the solution. Unithiol oxidation process study can give additional information about the outer factor effect on oxidation and reduction processes in a living body.

Keywordsdone physical factors, unithiol test, ESI mass-spectrometric study, associate
Received08.10.2018
Publication date10.10.2018
Number of characters1125
Cite   Download pdf To download PDF you should sign in
Размещенный ниже текст является ознакомительной версией и может не соответствовать печатной

views: 1352

Readers community rating: votes 0

1. Sokolovskij V.V. Tioldisul'fidnaya sistema v reakcii or-ganizma na faktory okruzhayushchej sredy [Tioldisulfidny system in reaction of an organism to environment factors]. Saint-Petersburg, Nauka Publ., 2008. 112 p. (In Russ.).

2. Jocelyn P. Biochemistry of the SH-group. The occurrence, chemical properties, metabolism and biological function of thiol and disulphides. London, N.Y., Acad. Press, 1972. 404 p.

3. Essex D. Redox control of platelet function. Antioxidants and redox signaling, 2009, vol. 11, no. 5, pp. 1191–1225.

4. Daynes R., Enioutina E., Jones D. Role of redox imbal-ance in the molecular mechanisms responsible for immu-nosenescence. Antioxidants and redox signaling, 2003, vol. 5, no. 5, pp. 537–548.

5. Sokolovskij V.V. [Tiolovy antioxidants in molecular me-chanisms of nonspecific reaction of an organism to ex-treme influence]. Voprosy medicinskoj himii [Questions of medical chemistry], 1988, vol. 34, no. 6, pp. 200–210. (In Russ.).

6. Sokolovskij V.V., Sokolovskaya T.M., Shubina L.N. [About the biochemical mechanism of toxic effect of nitro-gen dioxide]. Farmakologiya i toksikologiya [Pharmacolo-gy and toxicology], 1974, vol. 37, no. 4, pp. 469–471. (In Russ.).

7. Gorshkov E.S., Ivanov V.V., Sokolovskij V.V. Redoks reakcii v kosmobiologii [Redox reactions in a kosmobiolo-gy], Saint-Petersburg, Polytechnical university, 2014. 194 p. (In Russ.).

8. Kuleshov D.O., Berezkina T.E., Russkikh Y.V., et al. Ap-plication of high-performance liquid chromatography / high-resolution mass spectrometry to the investigation of sodium 2, 3-disulfanyl-1-propanesulfonate (unithiol) and products of its oxidation with hydrogen peroxide. Journal of Analytical Chemistry, 2016, vol. 71, no. 14, pp. 1381– 1388. Doi: 10.1134/S1061934816140082.

Система Orphus

Loading...
Up